Five-time World Champions Brazil are unbeaten in the tournament so far, after successfully negotiating 'Group of Death' Group G.
Dunga's side saw off North Korea 2-1 in their opener before sealing their qualification with an impressive 3-1 victory over Ivory Coast.
While the gloss was taken off their group campaign somewhat with the dour goalless stalemate with Portugal last time, the point at least helped Brazil finish top of their group.
They will also take confidence from the fact that they beat their opponents twice - a 3-0 win in Chile before a 4-2 home success - when heading the South America qualification group for the tournament, with Chile a point behind in second.
But it will not be easy at Johannesburg's Ellis Park against a Chilean side who also began their World Cup group campaign with two wins.
These came in Group H against Honduras and Switzerland - with a single goal in each game enough to decide the issue in their favour.
But Marcelo Bielsa's side then also suffered a hiccup in their last group game, going down 2-1 to well-fancied Spain.
Their cause was not helped by Marco Estrada's dismissal, while rash goalkeeping by Claudio Bravo gifted David Villa the opening goal, and Bielsa will be demanding an improvement.
Brazil will be boosted by the return of star man Kaka from suspension, while midfielder Elano has been declared fit after shrugging off a knock on his ankle.
The former Manchester City player, who scored in both of Brazil's opening two matches but missed the Portugal draw, took part in full training on Saturday and a team spokesperson confirmed he is available to play.
City forward Robinho also missed the bore draw but he too trained in the session and should feature against Chile.
But Felipe Melo remains a concern after twisting an ankle against Portugal, while Julio Baptista, who hurt his knee in the same game, is also a doubt.
Chile will be without Estrada after the midfielder received his marching orders against Spain following a second yellow card for clipping Fernando Torres.
But Chile will be able to cover his absence as fellow midfielders Carlos Carmona and Matias Fernandez missed the Spanish defeat through suspension and come back into consideration.
Along with Estrada, Jorge Valdivia came into the side last time to cover for the pair and he may again have to settle for a place on the bench.
Upfront, Mark Gonzalez was given the nod in Chile's attack last time ahead of Humberto Suazo after his headed winner against Switzerland but Bielsa may decide to change things around.